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ELK Biotechnology
SKU:ELK007ES
EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit
EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit
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$558.00 USD
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Alternative Names: GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
Assay Type: Sandwich
Sensitivity: 6.8 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Cardiovascular biology;Neuro science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 6.8 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Cardiovascular biology;Neuro science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.
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