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ELK Biotechnology
SKU:ELK0033
Human SMS1(Sphingomyelin Synthase 1) ELISA Kit
Human SMS1(Sphingomyelin Synthase 1) ELISA Kit
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Alternative Names: hmob33; Medulla oblongata-derived protein; MOB; MOB1; Phosphatidylcholine:ceramide cholinephosphotransferase 1; Protein Mob; SGMS1; SMS1; Sphingomyelin Synthase 1; TMEM23; Transmembrane protein 23
Assay Type: Sandwich
Sensitivity: 14.2 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMS1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q86VZ5
Assay Type: Sandwich
Sensitivity: 14.2 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMS1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q86VZ5
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