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ELK Biotechnology
SKU:ELK002ES
EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit
EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit
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Alternative Names: CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
Assay Type: Sandwich
Sensitivity: 7.5 pg/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Tumor immunity;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06731
Assay Type: Sandwich
Sensitivity: 7.5 pg/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Tumor immunity;Gastroenterology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06731
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