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ELK Biotechnology
SKU:ELK0027
Rat CREM(cAMP Responsive Element Modulator) ELISA Kit
Rat CREM(cAMP Responsive Element Modulator) ELISA Kit
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Alternative Names: Inducible cAMP early repressor; ICER
Assay Type: Sandwich
Sensitivity: 26.3 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CREM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CREM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CREM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CREM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q03061
Assay Type: Sandwich
Sensitivity: 26.3 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CREM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CREM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CREM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CREM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q03061
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