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ELK Biotechnology
SKU:ELK0001
Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit
Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit
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Alternative Names: SREBP2; SREBP-2; Srebf2_retired
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q3U1N2
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q3U1N2
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