ELISA Kit for Xeroderma Pigmentosum, Complementation Group F (XPF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.061ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids.

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group F (XPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group F (XPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group F (XPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group F (XPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ERCC4; RAD1; Excision Repair Cross-Complementing Rodent Repair Deficiency,Complementation 4; DNA repair protein complementing XP-F cells; DNA excision repair ERCC-4

Item Name

Xeroderma Pigmentosum, Complementation Group F

Research Area

Developmental science;

Reference

The age-related expression decline of ERCC1 and XPF for forensic age estimation: A preliminary study;