ELISA Kit for Von Willebrand Factor (vWF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

9.88-800ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Canis familiaris; Canine (Dog)

Sensitivity

The minimum detectable dose of this kit is typically less than 3.84ng/mL

Sample type

Plasma

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample.

Alternative Names

F8VWF; VWD; von Willebrand antigen 2

Item Name

Von Willebrand Factor

Research Area

Signal transduction;Hematology;

Reference