ELISA Kit for Vitamin K1 (VK1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 51.5pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin K1 (VK1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin K1 (VK1) and unlabeled Vitamin K1 (VK1) (Standards or samples) with the pre-coated antibody specific to Vitamin K1 (VK1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin K1 (VK1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin K1 (VK1) in the sample.

Alternative Names

Phylloquinone; Phytonadione; Phytomenadione

Item Name

Vitamin K1

Research Area

Hematology;Bone metabolism;

Reference