ELISA Kit for Vitamin D3 (VD3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.91ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin D3 (VD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin D3 (VD3) and unlabeled Vitamin D3 (VD3) (Standards or samples) with the pre-coated antibody specific to Vitamin D3 (VD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin D3 (VD3) in the sample.

Alternative Names

Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

Item Name

Vitamin D3

Research Area

Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;

Reference

Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats;Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats;Deficiencia de vitamina D en madres y neonatos mexicanos;P38/TRHr-Dependent Regulation of TPO in Thyroid Cells Contributes to the Hypothyroidism of Triclosan-Treated Rats;