ELISA Kit for Vitamin C (VC)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

493.8-40,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 192.1ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin C (VC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin C (VC) and unlabeled Vitamin C (VC) (Standards or samples) with the pre-coated antibody specific to Vitamin C (VC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin C (VC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin C (VC) in the sample.

Alternative Names

AA; L-Ascorbic Acid; Ascorbate

Item Name

Vitamin C

Research Area

Metabolic pathway;Infection immunity;Nutrition metabolism;

Reference

Salivary 1, 5-Anhydroglucitol and Vitamin Levels in Relation to Caries Risk in Children;