ELISA Kit for Vitamin B7 (VB7)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

32-20,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 13pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique.BSA conjugated VB7 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between BSA conjugated VB7 and unlabeled VB7 (Standards or samples) with HRP conjugated Streptavidin. After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of VB7 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VB7 in the sample.

Alternative Names

Biotin; Vitamin H; Coenzyme R; Biopeiderm

Item Name

Vitamin B7

Research Area

Reference