ELISA Kit for Vitamin B2 (VB2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

7.41-600ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 2.61ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin B2 (VB2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin B2 (VB2) and unlabeled Vitamin B2 (VB2) (Standards or samples) with the pre-coated antibody specific to Vitamin B2 (VB2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B2 (VB2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B2 (VB2) in the sample.

Alternative Names

Riboflavin; Riboflavine

Item Name

Vitamin B2

Research Area

Reference

Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial …;