ELISA Kit for Vanillylmandelic Acid (VMA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 43.7ng/mL

Sample type

urine and biological agents

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vanillylmandelic Acid (VMA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vanillylmandelic Acid (VMA) and unlabeled Vanillylmandelic Acid (VMA) (Standards or samples) with the pre-coated antibody specific to Vanillylmandelic Acid (VMA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vanillylmandelic Acid (VMA) in the sample.

Alternative Names

Vanillyl Mandelic Acid

Item Name

Vanillylmandelic Acid

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;

Reference