ELISA Kit for Urotensin 2 (UST2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.96pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Urotensin 2 (UST2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Urotensin 2 (UST2) and unlabeled Urotensin 2 (UST2) (Standards or samples) with the pre-coated antibody specific to Urotensin 2 (UST2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Urotensin 2 (UST2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Urotensin 2 (UST2) in the sample.

Alternative Names

UII; U-II; UCN2; PRO1068; Urotensin-II

Item Name

Urotensin 2

Research Area

Endocrinology;Neuro science;Hormone metabolism;

Reference

Serum Urotensin II Levels in Patients with Non-Dipper Hypertension;Urotensin II (U-II), a novel cyclic peptide, possibly associated with the pathophysiology of osteoarthritis;Urotensin-II level and its association with oxidative stress in early diabetic nephropathy.;Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects;