ELISA Kit for Urinary Trypsin Inhibitor (UTI)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.47-30ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.22ng/mL

Sample type

Serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urinary Trypsin Inhibitor (UTI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urinary Trypsin Inhibitor (UTI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urinary Trypsin Inhibitor (UTI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urinary Trypsin Inhibitor (UTI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

Ulinastatin; Bikunin

Item Name

Urinary Trypsin Inhibitor

Research Area

Reference

Urinary trypsin inhibitor levels are reduced in cerebrospinal fluid of multiple sclerosis and neuromyelitis optica patients during relapse;Excessive iodine intake, water chemicals and endemic goitre in a Sudanese coastal area.;