ELISA Kit for Ubiquitin (Ub)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

4.94-400ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.92ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ubiquitin (Ub) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ubiquitin (Ub) and unlabeled Ubiquitin (Ub) (Standards or samples) with the pre-coated antibody specific to Ubiquitin (Ub). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ubiquitin (Ub) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ubiquitin (Ub) in the sample.

Alternative Names

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

Item Name

Ubiquitin

Research Area

Reproductive science;Neuro science;Developmental science;

Reference