ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

125-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 50pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14

Item Name

Tumor Necrosis Factor Receptor Superfamily, Member 12A

Research Area

CD & Adhesion molecule;Tumor immunity;

Reference