ELISA Kit for Triglyceride (TG)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.9-1,000ug/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.8ug/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Triglyceride (TG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Triglyceride (TG) and unlabeled Triglyceride (TG) (Standards or samples) with the pre-coated antibody specific to Triglyceride (TG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Triglyceride (TG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Triglyceride (TG) in the sample.

Alternative Names

TAG; Triacylglycerol; Triacylglyceride

Item Name

Triglyceride

Research Area

Metabolic pathway;Cardiovascular biology;Hepatology;Nutrition metabolism;

Reference

Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.;Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle;