ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.67pg/mL

Sample type

serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transforming Growth Factor Beta 1 (TGFb1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transforming Growth Factor Beta 1 (TGFb1) and unlabeled Transforming Growth Factor Beta 1 (TGFb1) (Standards or samples) with the pre-coated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transforming Growth Factor Beta 1 (TGFb1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transforming Growth Factor Beta 1 (TGFb1) in the sample.

Alternative Names

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

Item Name

Transforming Growth Factor Beta 1

Research Area

Cytokine;Tumor immunity;Infection immunity;

Reference