ELISA Kit for Tight Junction Associated Protein 1, Peripheral (TJAP1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

78-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 34pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Associated Protein 1, Peripheral (TJAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tight Junction Associated Protein 1, Peripheral (TJAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tight Junction Associated Protein 1, Peripheral (TJAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tight Junction Associated Protein 1, Peripheral (TJAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PILT; TJP4; Tight Junction Protein 4; Protein incorporated later into tight junctions

Item Name

Tight Junction Associated Protein 1, Peripheral

Research Area

Signal transduction;

Reference