ELISA Kit for Thyrotropin Releasing Hormone (TRH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Sus scrofa; Porcine (Pig)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.93pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyrotropin Releasing Hormone (TRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyrotropin Releasing Hormone (TRH) and unlabeled Thyrotropin Releasing Hormone (TRH) (Standards or samples) with the pre-coated antibody specific to Thyrotropin Releasing Hormone (TRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyrotropin Releasing Hormone (TRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyrotropin Releasing Hormone (TRH) in the sample.

Alternative Names

TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor

Item Name

Thyrotropin Releasing Hormone

Research Area

Endocrinology;Neuro science;Hepatology;Hormone metabolism;

Reference