ELISA Kit for Thymic Factor (TF)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

37.0-3,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 13.3pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymic Factor (TF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymic Factor (TF) and unlabeled Thymic Factor (TF) (Standards or samples) with the pre-coated antibody specific to Thymic Factor (TF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymic Factor (TF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymic Factor (TF) in the sample.

Alternative Names

FTS; Facteur Thymique Serique; Thymulin

Item Name

Thymic Factor

Research Area

Infection immunity;Endocrinology;Neuro science;Hormone metabolism;

Reference