ELISA Kit for Thioredoxin Related Protein 14kDa (TRP14)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.060ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Related Protein 14kDa (TRP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Related Protein 14kDa (TRP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Related Protein 14kDa (TRP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Related Protein 14kDa (TRP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

TXNDC17; TXNL5; Thioredoxin-Like 5; Thioredoxin Domain Containing 17

Item Name

Thioredoxin Related Protein 14kDa

Research Area

Hematology;

Reference