ELISA Kit for Tetrahydrobiopterin (THB)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

391-100,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 155pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tetrahydrobiopterin (THB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tetrahydrobiopterin (THB) and unlabeled Tetrahydrobiopterin (THB) (Standards or samples) with the pre-coated antibody specific to Tetrahydrobiopterin (THB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tetrahydrobiopterin (THB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tetrahydrobiopterin (THB) in the sample.

Alternative Names

BH4; Kuvan; Sapropterin

Item Name

Tetrahydrobiopterin

Research Area

Reference