ELISA Kit for Tachykinin, Precursor 1 (TAC1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 51.9pg/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tachykinin, Precursor 1 (TAC1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tachykinin, Precursor 1 (TAC1) and unlabeled Tachykinin, Precursor 1 (TAC1) (Standards or samples) with the pre-coated antibody specific to Tachykinin, Precursor 1 (TAC1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample.

Alternative Names

NK2; NKNA; NKA; TAC2; •PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide

Item Name

Tachykinin, Precursor 1

Research Area

Neuro science;

Reference