ELISA Kit for Synaptophysin (SYP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 90.2pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Synaptophysin (SYP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Synaptophysin (SYP) and unlabeled Synaptophysin (SYP) (Standards or samples) with the pre-coated antibody specific to Synaptophysin (SYP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Synaptophysin (SYP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Synaptophysin (SYP) in the sample.

Alternative Names

Major synaptic vesicle protein p38

Item Name

Synaptophysin

Research Area

Neuro science;

Reference