ELISA Kit for Surfactant Protein C (SP-C)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.625-40ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.241ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Protein C (SP-C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Surfactant Protein C (SP-C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Protein C (SP-C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Protein C (SP-C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SFTPC; PSP-C; SFTP2; SPC; SP5; Surfactant Associated Protein C; Pulmonary Surfactant Protein C; Pulmonary surfactant-associated proteolipid SPL(Val)

Item Name

Surfactant Protein C

Research Area

Tumor immunity;Infection immunity;Pulmonology;

Reference

Autophagy regulates hyperoxia-induced intracellular accumulation of surfactant protein C in alveolar type II cells;Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.;