ELISA Kit for Sphingosine-1-Phosphate (S1P)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.62ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sphingosine-1-Phosphate (S1P) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sphingosine-1-Phosphate (S1P) and unlabeled Sphingosine-1-Phosphate (S1P) (Standards or samples) with the pre-coated antibody specific to Sphingosine-1-Phosphate (S1P). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sphingosine-1-Phosphate (S1P) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sphingosine-1-Phosphate (S1P) in the sample.

Alternative Names

Item Name

Sphingosine-1-Phosphate

Research Area

Reference

Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling;SPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway;