ELISA Kit for Sphingomyelin Phosphodiesterase 3 (SMPD3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.117ng/mL

Sample type

tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingomyelin Phosphodiesterase 3 (SMPD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingomyelin Phosphodiesterase 3 (SMPD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingomyelin Phosphodiesterase 3 (SMPD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingomyelin Phosphodiesterase 3 (SMPD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

NSMASE2; Neutral Sphingomyelinase II

Item Name

Sphingomyelin Phosphodiesterase 3

Research Area

Enzyme & Kinase;

Reference