ELISA Kit for Sperm Adhesion Molecule 1 (SPAM1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.057ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sperm Adhesion Molecule 1 (SPAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sperm Adhesion Molecule 1 (SPAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sperm Adhesion Molecule 1 (SPAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sperm Adhesion Molecule 1 (SPAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

HYAL1; HYA1; HYAL3; HYAL5; PH-20; PH20; SPAG15; PH-20 Hyaluronidase; Zona Pellucida Binding

Item Name

Sperm Adhesion Molecule 1

Research Area

Reproductive science;

Reference