ELISA Kit for Small Breast Epithelial Mucin (SBEM)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.312-20ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.129ng/mL

Sample type

Serum, plasma, tissue homogenates, saliva and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Breast Epithelial Mucin (SBEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Breast Epithelial Mucin (SBEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Breast Epithelial Mucin (SBEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Breast Epithelial Mucin (SBEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

MUCL1; Mucin-Like Protein 1; Protein BS106; Mucin-like protein 1

Item Name

Small Breast Epithelial Mucin

Research Area

Tumor immunity;Infection immunity;Reproductive science;

Reference