ELISA Kit for SMAD family member 9 (SMAD9)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.061ng/mL

Sample type

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SMAD family member 9 (SMAD9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SMAD family member 9 (SMAD9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SMAD family member 9 (SMAD9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SMAD family member 9 (SMAD9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

MADH2; MADR2; Madh6; Mothers against DPP homolog 9; Mothers Against Decapentaplegic Homolog 9

Item Name

SMAD family member 9

Research Area

Signal transduction;Developmental science;Bone metabolism;

Reference