ELISA Kit for Skin Aspartic Protease (SASP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

31.2-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 12.1pg/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Skin Aspartic Protease (SASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Skin Aspartic Protease (SASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Skin Aspartic Protease (SASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Skin Aspartic Protease (SASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

ASPRV1; Taps; SASPase; MUNO; Aspartic Peptidase,Retroviral-Like 1; Skin-specific retroviral-like aspartic protease; TPA-inducible aspartic proteinase-like protein

Item Name

Skin Aspartic Protease

Research Area

Enzyme & Kinase;

Reference