ELISA Kit for Serine Peptidase Inhibitor Kazal Type 1 (SPINK1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.059ng/mL.

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 1 (SPINK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 1 (SPINK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 1 (SPINK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PCTT; PSTI; TATI; Tumor-Associated Trypsin Inhibitor; Pancreatic secretory trypsin inhibitor; Tumor-associated trypsin inhibitor

Item Name

Serine Peptidase Inhibitor Kazal Type 1

Research Area

Tumor immunity;

Reference