ELISA Kit for Selenoprotein P1, Plasma (SEPP1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.78-50ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.33ng/mL

Sample type

serum, plasma and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenoprotein P1, Plasma (SEPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenoprotein P1, Plasma (SEPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenoprotein P1, Plasma (SEPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenoprotein P1, Plasma (SEPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

SEPP; SeP; SEP-P1; SELP

Item Name

Selenoprotein P1, Plasma

Research Area

Metabolic pathway;Infection immunity;

Reference

Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis;Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease;Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia;Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism;Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance.;Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans.;DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism.;Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome;Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study;Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth;Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury;Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits;Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus;Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus;Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children?;