ELISA Kit for Secretory Immunoglobulin A (sIgA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

617.28-50000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Chicken (Gallus)

Sensitivity

The minimum detectable dose of this kit is typically less than 248.5pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretory Immunoglobulin A (sIgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretory Immunoglobulin A (sIgA) and unlabeled Secretory Immunoglobulin A (sIgA) (Standards or samples) with the pre-coated antibody specific to Secretory Immunoglobulin A (sIgA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample.

Alternative Names

S-IgA

Item Name

Secretory Immunoglobulin A

Research Area

Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;

Reference

Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88;Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens;