ELISA Kit for Secretory Immunoglobulin A (sIgA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Canis familiaris; Canine (Dog)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.51ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Immunoglobulin A (sIgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretory Immunoglobulin A (sIgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretory Immunoglobulin A (sIgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretory Immunoglobulin A (sIgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

S-IgA

Item Name

Secretory Immunoglobulin A

Research Area

Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;

Reference