ELISA Kit for Salusin Alpha (SALa)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.86pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Salusin Alpha (SALa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Salusin Alpha (SALa) and unlabeled Salusin Alpha (SALa) (Standards or samples) with the pre-coated antibody specific to Salusin Alpha (SALa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Salusin Alpha (SALa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Salusin Alpha (SALa) in the sample.

Alternative Names

Item Name

Salusin Alpha

Research Area

Infection immunity;Endocrinology;Cardiovascular biology;Neuro science;

Reference