ELISA Kit for S-Adenosyl Methionine (SAM)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.84ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to S-Adenosyl Methionine (SAM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled S-Adenosyl Methionine (SAM) and unlabeled S-Adenosyl Methionine (SAM) (Standards or samples) with the pre-coated antibody specific to S-Adenosyl Methionine (SAM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of S-Adenosyl Methionine (SAM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of S-Adenosyl Methionine (SAM) in the sample.

Alternative Names

SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride

Item Name

S-Adenosyl Methionine

Research Area

Reference

Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation;Attenuated expression of MTR in both prenatally androgenized mice and women with the hyperandrogenic phenotype of PCOS;Metabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation;