ELISA Kit for Resveratrol (RVT)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 89.3ng/mL

Sample type

Plant tissue and biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Resveratrol (RVT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Resveratrol (RVT) and unlabeled Resveratrol (RVT) (Standards or samples) with the pre-coated antibody specific to Resveratrol (RVT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Resveratrol (RVT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Resveratrol (RVT) in the sample.

Alternative Names

trans-3,5,4'-Trihydroxystilbene; 3,4',5-Stilbenetriol; trans-Resveratrol

Item Name

Resveratrol

Research Area

Reference

Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study;