ELISA Kit for Relaxin 3 (RLN3)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.46-10000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 43.6pg/mL

Sample type

Serum, plasma and other biological fluids.

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Relaxin 3 (RLN3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Relaxin 3 (RLN3) and unlabeled Relaxin 3 (RLN3) (Standards or samples) with the pre-coated antibody specific to Relaxin 3 (RLN3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Relaxin 3 (RLN3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Relaxin 3 (RLN3) in the sample.

Alternative Names

ZINS4; RXN3; H3; INSL7; Insulin-like peptide 7; Prorelaxin H3

Item Name

Relaxin 3

Research Area

Endocrinology;

Reference