ELISA Kit for Ras Guanyl Releasing Protein 2 (RASGRP2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

0.156-10ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.057ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Guanyl Releasing Protein 2 (RASGRP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Guanyl Releasing Protein 2 (RASGRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Guanyl Releasing Protein 2 (RASGRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Guanyl Releasing Protein 2 (RASGRP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

CALDAG-GEFI; CDC25L, MCG7; Calcium and DAG-regulated guanine nucleotide exchange factor I; Cdc25-like protein; F25B3.3 kinase-like protein

Item Name

Ras Guanyl Releasing Protein 2

Research Area

Tumor immunity;

Reference