ELISA Kit for Pyruvate kinase isozymes R/L (PKLR)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.62ng/mL

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate kinase isozymes R/L (PKLR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate kinase isozymes R/L (PKLR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate kinase isozymes R/L (PKLR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate kinase isozymes R/L (PKLR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

PK; PyK; PK1; PKL; Pyruvate Kinase Isozymes R/L; Glutamic--pyruvic transaminase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase

Item Name

Pyruvate kinase isozymes R/L

Research Area

Enzyme & Kinase;Metabolic pathway;

Reference