ELISA Kit for Pyruvate Dehydrogenase Complex Component X (PDHX)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.56-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.54ng/mL

Sample type

Tissue homogenates, cell lysates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Complex Component X (PDHX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Complex Component X (PDHX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Complex Component X (PDHX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Complex Component X (PDHX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

E3BP; proX; PDX1; OPDX; DLDBP; E3-binding protein; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex

Item Name

Pyruvate Dehydrogenase Complex Component X

Research Area

Enzyme & Kinase;

Reference