ELISA Kit for Prostaglandin D2 (PGD2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.61pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGD2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGD2 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGD2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGD2 in the sample.

Alternative Names

PG-D2

Item Name

Prostaglandin D2

Research Area

Reference

Effect of the PGD2-DP signaling pathway on primary cultured rat hippocampal neuron injury caused by aluminum overload;Effects of icariin on asthma mouse model are associated with regulation of prostaglandin D2 level;Oleanolic acid protects against cognitive decline and neuroinflammation-mediated neurotoxicity by blocking secretory phospholipase A2 IIA-activated calcium signals;