ELISA Kit for Prostacyclin (PGI2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.93pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to prostacyclin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled prostacyclin analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of prostacyclin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of prostacyclin in the sample.

Alternative Names

prostaglandin I2

Item Name

Prostacyclin

Research Area

Cardiovascular biology;Hormone metabolism;

Reference

Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome;Comparison of the Effects of Indobufen and Warfarin in a Rat Model of Adenine-Induced Chronic Kidney Disease;