ELISA Kit for Prolactin (PRL)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.12-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.39ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin (PRL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin (PRL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin (PRL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolactin (PRL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Alternative Names

LTH; Luteotropic Hormone

Item Name

Prolactin

Research Area

Endocrinology;Reproductive science;Genetic science;Hormone metabolism;

Reference

Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves;The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway;