ELISA Kit for Proinsulin (PI)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human)

Sensitivity

The minimum detectable dose of this kit is typically less than 5.25pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Proinsulin (PI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Proinsulin (PI) and unlabeled Proinsulin (PI) (Standards or samples) with the pre-coated antibody specific to Proinsulin (PI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Proinsulin (PI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proinsulin (PI) in the sample.

Alternative Names

INS; Pro-Insulin; Preproinsulin

Item Name

Proinsulin

Research Area

Endocrinology;Hormone metabolism;

Reference