ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

2.47-200ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.93ng/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen II C-Terminal Propeptide (PIICP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen II C-Terminal Propeptide (PIICP) and unlabeled Procollagen II C-Terminal Propeptide (PIICP) (Standards or samples) with the pre-coated antibody specific to Procollagen II C-Terminal Propeptide (PIICP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample.

Alternative Names

P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Item Name

Procollagen II C-Terminal Propeptide

Research Area

Infection immunity;Bone metabolism;Rheumatology;

Reference