ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

246.9-20,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 91.5pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen I N-Terminal Propeptide (PINP) and unlabeled Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen I N-Terminal Propeptide (PINP) in the sample.

Alternative Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Item Name

Procollagen I N-Terminal Propeptide

Research Area

Metabolic pathway;Hepatology;Bone metabolism;

Reference

The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.;Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy;Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy;The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats;The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism;Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction;